Upcoming project: Production of non-hazardous feed from dry-resistant Leguminose

MENDELU internal grant Proof of concept 2 has supported our project:

Production of non-hazardous feed from dry-resistant Leguminose

The aim of the MENDELU Proof of concept 2 is an efficient system of support of the proof-of-concept activities (hereafter PoC) at MENDELU in 2020 – 2022. The proposal of the PoC suppport is drafted as an integral part of the commercialisation system developer at the university. Regarding the PoC, MENDELU expects efficient transformation of its research and development results into practical applications, enabling their real commercial exploitation and related use in practice. More precisely in common business or other activity of end-user of technologies.

The main investigator is Assoc. Prof. Pavel Knot, Ph.D.

About the project:

Mycotoxins and biogenic amines are two types of food contaminants that can cause health problems in animals. Mycotoxins are toxic substances produced by certain fungi that can grow on food crops, while biogenic amines are naturally occurring compounds that can be found in fermented feed such as silage. To reduce the risk of exposure to these contaminants, it is important to properly store and handle food products. Additionally, regular testing and monitoring of food products can help to identify and prevent the presence of mycotoxins and biogenic amines.

Just Released in the Revista Brasileira de Zootecnia

Degradation of biogenic amines and in vitro evaluation of ruminal parameters of the ruminal fluid of Charolais sheep

by Ivana Kolackova, Jiri Skladanka, Sylvie Skalickova, Pavel Horky, Natalia Cernei, Zuzana Lackova, Jiri Trinacty, Vojtech Adam


In this study, we reported the evaluation of ruminal parameters and the rate and degree of degradation of biogenic amines (BA) in vitro, using ruminal fluid from sheep fed two different diets (meadow hay and silage of Medicago sativa L.). Herein, the Charolais sheep breed with a ruminal cannula was used to gather samples. Multiple rumen parameters were evaluated after 10 days of the feeding period. Samples were processed using fermenters inoculated by BA (histamine, tyramine, putrescine, cadaverine, spermine, and spermidine) during 8-h fermentation. Levels of BA were determined using ion-exchange chromatography, and volatile fatty acids (VFA) were determined using gas chromatography. More rapid BA degradation was noticed in hay than in haylage. Overall, VFA levels increased with duration of the fermentation, while pH lowered. The rapid increase was observed after 4 h of fermentation. Ammonia concentration had a decreasing trend in silage and increasing in the hay. Hypotheses on BA degradation were accepted. Important knowledge of the development of ruminal parameters in time was obtained, and based on gathered information, it is possible to say that other parameters and their impacts on BA in rumen need to be examined further.

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